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US 10,369,238 B2Granted
Method for sterilizing membrane comprising glucose oxidase and associated bio-sensor
AssigneeGeneral Electric Company
InventorsYida Xu, Rui Chen, Lin Chen
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Abstract
A method for sterilizing a membrane comprising an oxidoreductase enzyme, comprises: irradiating with gamma radiation the membrane comprising an oxidoreductase enzyme soaked in an aqueous buffer solution. Associated biosensors and bioreactors are also described.
Claims20
A method for sterilizing a membrane comprising an oxidoreductase enzyme, the method comprising:
- irradiating with gamma radiation the membrane comprising the oxidoreductase enzyme soaked in a phosphate buffer saline (PBS) buffer solution,
- wherein the PBS buffer consists essentially of sodium phosphate dibasic, sodium acid phosphate, sodium chloride, glycine, N, N′-ethanediybis (N-carboxymethyl) dipotassium, sodium benzoate, and gentamicin sulphate in water.
A method for sterilizing a membrane comprising glucose oxidase, the method comprising:
- making a phosphate buffer saline (PBS) solution by dissolving a PBS buffer powder in water,
- wherein the PBS buffer powder consists essentially of 50-55% wt sodium phosphate dibasic, 10-15% wt sodium acid phosphate, 16-22% wt sodium chloride, less than 10% wt glycine and N, N′-ethanediylbis (N-carboxymethyl) dipotassium, less than 10% wt sodium benzoate, and less than 1% wt gentamicin sulphate, and
- irradiating with gamma radiation the membrane comprising glucose oxidase soaked in a phosphate buffer saline (PBS) solution,
- wherein the gamma radiation is carried out in a range of from about 25 kGy to about 40 kGy at rate of about 20 kGy/hour.
1 more element
A biosensor comprising a sterilized membrane that comprises glucose oxidase, wherein the membrane is sterilized by:
- irradiating with gamma radiation while the membrane comprising glucose oxidase is soaked in a phosphate buffer saline (PBS) solution,
- wherein the PBS buffer is made by dissolving a PBS powder in water, wherein the PBS buffer powder consists essentially of 50-55% wt sodium phosphate dibasic, 10-15% wt sodium acid phosphate, 16-22% wt sodium chloride, less than 10% wt glycine and N, N′-ethanediylbis (N-carboxymethyl) dipotassium, less than 10% wt sodium benzoate, and less than 1% wt gentamicin sulphate, and
- wherein the gamma radiation is carried out in a range of from about 25 kGy to about 40 kGy at rate of about 20 kGy/hour.
Dependencies are inferred from the claim text.