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US 10,369,235 B2Granted
Non-diffusive botulinum toxin causing local muscle paralysis, and purification method thereof
AssigneeMedexgen Co., Ltd.
InventorsYong Hoon Chung, Hyun Sub Lee
Life of this patent
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Abstract
The present invention relates to a method for purifying a non-spreading botulinum toxin that causes local muscle paralysis and a non-spreading botulinum toxin obtained thereby. The method comprises the steps of: subjecting a purified botulinum toxin type A product to ion-exchange chromatography using a controlled pH of buffer, concentration of sodium chloride (NaCl), thereby separating the botulinum toxin type A product into subfractions; and collecting a subfraction having an A260/A280 value in a specific range from the separated subfractions.
Claims3
A method for determining a non-spreading botulinum toxin, comprising:
- injecting the non-spreading botulinum toxin into the either left or right hind limb calf muscle of mouse (4-6 wk old, weighing 18-22 g) in an amount equivalent to 1.5-3 times the LD50 of the toxin in the volume ranges of 20-50 μL;
- and
- assessing paralysis conditions of the hind limb muscles and respiratory muscles of the injected mouse and a survival rate of the injected mouse to determine a non-spreading botulinum toxin.
Dependencies are inferred from the claim text.