Influenza H5 vaccines
OwnerMauricio Realpe-Quintero, Paulino Carlos Gonzalez-Hernandez +1inventors
InventorsMauricio Realpe-Quintero, Paulino Carlos Gonzalez-Hernandez, Eric Vaughn
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Abstract
The present invention is based on the surprising finding that H5 protein of clade 1 H5N1 induces, in particular by a single-shot vaccination, a cross-clade protective immune response to influenza viruses with H5N1 HA. In one aspect, the invention is thus directed to H5 protein of clade 1 H5N1 virus for use in a method of treating or preventing infections with H5N1 virus of a different clade, namely of a clade different from clade 1 or from any clade with the exception of clade 1, respectively.
Claims29
A method for protecting an animal against a clade 2 H5N1 influenza virus infection, comprising:
- administering to the animal a composition comprising (i) a crude whole cell H5 protein of a clade 1 H5N1 influenza virus, expressed in insect cells by a baculovirus based expression system, in an amount effective to induce a cross-clade protective immune response in the animal against the clade 2 H5N1 influenza virus infection, and (ii) an inactivated Newcastle disease virus;
- wherein the H5 protein of the clade 1 H5N1 influenza virus comprises a polypeptide sequence that has at least 98% sequence identity to SEQ ID NO:1;
- wherein the H5 protein of the clade 1 H5N1 influenza virus has amino acid 223N and modification 328K+;
- wherein amino acid position numbering of the H5 protein of the clade 1 H5N1 influenza virus refers to amino acid positions as given in SEQ ID NO:2;
- and
- wherein modification 328K+ means that a second Lysine (K+) is inserted at amino acid position 328 of the H5 protein of the clade 1 H5N1 influenza virus.
A method for protecting an animal against clade 2 H5N1 influenza virus infection, comprising:
- administering to the animal a vaccine comprising (i) an H5 protein of a clade 1 H5N1 influenza virus in an amount effective to induce a cross-clade protective immune response in the animal against clade 2 H5N1 influenza virus infection, (ii) an inactivated Newcastle disease virus, and (iii) an adjuvant,
- wherein the H5 protein of the clade 1 H5N1 influenza virus comprises a polypeptide sequence that has at least 98% sequence identity to SEQ ID NO:1,
- wherein amino acid position numbering of the H5 protein of the clade 1 H5N1 influenza virus refers to amino acid positions as given in SEQ ID NO:2;
- and
- wherein modification 328K+ means that a second Lysine (K+) is inserted at amino acid position 328 of the H5 protein of the clade 1 H5N1 influenza virus.
Dependencies are inferred from the claim text.