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US 10,369,173 B2Granted
Optimized methods for generation of cardiac stem cells from cardiac tissue and their use in cardiac therapy
AssigneeCapricor, Inc.
InventorsMichelle Kreke, Rachel Smith
Life of this patent
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Abstract
The present disclosure relates generally to methods for the increased processing of tissue for the generation of cardiac stem cells, wherein the stem cells are suitable for use in cardiac stem cell therapy. In particular, several embodiments relate to the processing of allogeneic donor cardiac tissue for the generation of multiple patient doses of cardiac stem cells.
Claims20
A method for preparing filtered cardiosphere-derived cells (CDCs) suitable for allogeneic cardiac stem cell therapy, comprising:
- receiving donor cardiac tissue from a subject;
- processing said piece of donor cardiac tissue into a plurality of tissue explants ranging from about 200 μm3 to about 600 μm3, wherein said explants range from about 0.3 to about 0.6 grams;
- enzymatically digesting said explants for a time ranging from about 30 seconds to about 5 minutes;
- culturing said explants at a density of about 1 explant per 400 to 700 cm2 culturing surface area until cells migrate from said explant;
- collecting the cells that migrate from the explant;
- culturing the collected cells in order to generate CDCs;
- harvesting the CDCs;
- and
- filtering said harvested CDCs to remove particles greater than 50 μm in diameter to isolate CDCs less than 50 μm in diameter to facilitate allogeneic cardiac stem cell therapy.
6 more elements
A method for preparing cardiosphere derived cells (CDCs), comprising:
- processing cardiac tissue into a plurality of tissue explants;
- culturing said explants until cells migrate from said explant;
- collecting the cells that migrate from the explant;
- culturing the collected cells in order to generate CDCs;
- harvesting the CDCs;
- filtering said harvested CDCs remove particles greater than about 50 μm.
3 more elements
Dependencies are inferred from the claim text.