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AssigneesTokyo Women's Medical University, The Jikei University, Cellseed Inc.
InventorsTakanori Hama, Hiromi Kojima, Hiroshi Moriyama, Masayuki Yamato, Teruo Okano
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The purpose of the present invention is to obtain an alternative to a substitute of the mucosa in the middle ear which is engrafted on the surface of the bone in the middle ear, hyperplasia of the granulation tissue and the bone and the development of the fibroblast cells in the middle ear are suppressed, and to obtain a middle ear mucosa-like cell sheet retaining cilia in the surface layer, comprising culturing nasal epithelium cells on a cell culture substrate coated with a polymer whose hydration force changes within a temperature range of 0 to 80° C., wherein the cells are cultured within a temperature range where the hydration force of the polymer is weak, and then changing the temperature to a temperature at which the hydration force is strong to recover the cultured cell sheet.
A method of transplanting a middle ear mucosa-like cell sheet into a subject in need thereof, the method comprising
A method of preparing a middle ear mucosa-like cell sheet containing nasal epithelium cells to be transplanted into a subject in need thereof, the middle ear mucosa-like cell sheet containing nasal epithelium cells retaining cilia thereof and nasal mucosa cells, the nasal epithelium cells originating from inferior turbinate mucosa, middle turbinate mucosa, or mucosa in paranasal sinus, wherein the nasal epithelium cells in the middle ear mucosa-like cell sheet comprise flat forms of the nasal epithelium cells, said method comprising:
Dependencies are inferred from the claim text.