Cell collection devices

A method for collecting cells from an anus of a patient, the method comprising:

• obtaining a cell collection device comprising a sample brush including an elongated shaft having a distal end and radially extending bristles provided at the distal end and a tubular outer sleeve through which the elongated shaft extends, the outer sleeve including a distal end including a distal tip having a distal opening from which the distal end of the elongated shaft and its bristles extend;
• inserting the distal end of a sample brush extending from the distal tip of the outer sheath into the anus;
• rotating the sample brush while the distal end of the brush is within the anus to collect cells with bristles provided at the distal end of the brush;
• withdrawing the sample brush from the anus;
• linearly moving the outer sleeve relative to the sample brush or vice versa such that the bristles of the brush extending from the distal opening of the outer sleeve are retracted into the outer sleeve;
• inserting the distal end of the outer sleeve along with the bristles into a cell collection liquid;
• and
• rotating the sample brush relative to the outer sleeve or vice versa to enable the bristles of the brush to alternately extend from bristle windows positioned at the distal end of the outer sleeve and retract into the outer sleeve, wherein the extension and retraction of the bristles causes cells collected by the bristles to transfer to the cell collection liquid.