Identifying liquid blood components from sensed data to monitor specimen integrity

A system for setting one or more reference ranges for performing a pre-analysis of blood samples to detect specimen defects in an automated blood sample processing device, the system comprising:

• one or more sensors for capturing sensor data elements for one or more samples, the one or more sensors comprising a red color detector, a blue color detector, and a green color detector;
• a sample properties processor having one or more components for identifying reference sample properties, wherein the reference sample properties correspond to a testable criteria associated with a liquid blood component;
• and
• one or more computer storage media storing computer-useable instructions that, when used by the sample properties processor, cause the sample properties processor to perform operations comprising:
• capturing, by the one or more sensors, a first plurality of color elements for a first reference sample, wherein the first reference sample corresponds to the testable criteria associated with the liquid blood component;
• creating, by a linear array generator, a digital record of each color element in the first plurality of color elements by breaking the first plurality of color elements of the first reference sample into a first linear array using each color element in the first plurality of color elements;
• analyzing, by a background identifier, the first linear array from a first end of the first linear array to determine a first color element in the first plurality of color elements with a valid brightness range associated with the liquid blood component;
• analyzing, by the background identifier, the first linear array from a second end of the first linear array to determine a second color element in the first plurality of color elements with the valid brightness range associated with the liquid blood component;
• determining, by the background identifier, one or more background color elements from the first linear array, wherein the one or more background color elements are comprised of color elements in the first plurality of color elements that fall outside of the valid brightness range and removing the one or more background color elements from the first linear array to create a first clean array;
• determining, by a distance array generator, a distance between each color element of the first clean array and creating a first distance array;
• determining, by a transition indication array generator, if each distance of the first distance array is above a transition indication threshold, wherein a distance above the transition indication threshold indicates a color transition, and creating a first transition indication array comprising each color transition determined from the first distance array;
• determining, by a liquid portions determiner, one or more color arrays for the first reference sample by cutting the first transition indication array at the each color transition, the one or more color arrays for the first reference sample comprising one or more color elements of the plurality of color elements and identifying a first testable criteria color array that corresponds to the testable criteria for the first reference sample;
• determining, by a reference range determiner, based on the first testable criteria color array for the first reference sample, a first reference range boundary by determining a median of a first boundary region of the first reference sample, and creating a digital record of the first reference range boundary, wherein the first boundary region is comprised of a frequency of transition indications in the transition indication array that is above a predetermined frequency;
• capturing, by the one or more sensors, a second plurality of color elements for a second reference sample, wherein the second reference sample corresponds to the testable criteria;
• creating, by the linear array generator, a digital record of each color element in the second plurality of color elements by breaking the second plurality of color elements of the second reference sample into a second linear array using each color element in the second plurality of color elements;
• analyzing, by the background identifier, the second linear array from a first end of the second linear array to determine a first color element in the second plurality of color elements with the valid brightness range associated with the liquid blood component;
• analyzing, by the background identifier, the second linear array from a second end of the second linear array to determine a second color element in the second plurality of color elements with the valid brightness range associated with the liquid blood component;
• determining, by the background identifier, one or more background color elements from the second linear array,
• wherein the one or more background color elements are comprised of color elements in the second plurality of color elements that fall outside of the valid brightness range and removing the one or more background color elements from the second linear array to create a second clean array;
• determining, by the distance array generator, a distance between each color element of the second clean array and creating a second distance array;
• determining, by the transition indication array generator, if each distance of the second distance array is above the transition indication threshold;
• and creating a second transition indication array comprising each color transition determined from the second distance array;
• determining, by the liquid portions determiner, one or more color arrays for the second reference sample, the one or more color arrays for the second reference sample comprising one or more color elements of the second plurality of color elements and identifying a second testable criteria array that corresponds to the testable criteria for the second reference sample;
• determining, by the reference range determiner, based on the second testable criteria color array for the second reference sample, a second reference range boundary by determining a median of a second boundary region of the second reference sample, and creating a digital record of the second reference range boundary;
• and
• generating, by the reference range determiner, a reference range for the testable criteria, wherein the reference range comprises the first reference range boundary and the second reference range boundary.

A method for determining reference ranges and defects associated with blood samples in an automated blood sample processing system, the method comprising:

• capturing, by one or more sensors including a red color detector, a green color detector, and a blue color detector, a first plurality of images of a first reference sample, and a second plurality of images of a second reference sample;
• determining, a first plurality of color elements corresponding to one or more testable criteria associated with one or more liquid blood properties for the first reference sample and a second plurality of color elements for the second reference sample corresponding to one or more testable criteria associated with one or more liquid blood properties;
• creating, based on the first plurality of color elements, a first reference sample array and based on the second plurality of color elements, a second reference sample array;
• analyzing the first reference sample array and the second reference sample array from a first end, respectively, to determine a first color element in the first plurality of color elements and in the second plurality of color elements, respectively, with a valid brightness range associated with the one or more liquid blood properties;
• analyzing the first reference sample array and the second reference sample array from a second end, respectively, to determine a second color element in the first plurality of color elements and in the second plurality of color elements, respectively, with the valid brightness range associated with the one or more liquid blood properties;
• trimming all color elements from the first reference sample array and the second reference sample array that fall outside of the valid brightness range associated with the one or more liquid blood properties and storing the trimmed first reference sample array and trimmed second reference sample array as a first clean array and a second clean array, respectively;
• determining one or more color transitions for the first clean array and for the second clean array;
• cutting the first clean array and the second clean array at each color transition of the one or more color transitions;
• determining a distance between each color element in the first clean array and a distance between each color element in the second clean array and creating a first distance array and a second distance array, respectively;
• determining a first color transition indication array from the first distance array and a second color transition indication array from the second distance array, wherein each of the first color transition indication array and the second color transition indication array comprise at least one color transition, wherein a color transition is indicated when a distance in the first distance array and in the second distance array, respectively, is above a transition indication threshold;
• creating one or more color arrays for the first reference sample and one or more color arrays for the second reference sample by cutting the first color transition indication array and the second color transition indication array, respectively, at the at least one color transition, the one or more color arrays for the first reference sample and the one or more color arrays for the second reference sample having color values corresponding to one or more liquid blood properties, wherein the color values of the one or more color arrays for the first reference sample and the color values of the one or more color arrays for the second reference sample are determined by identifying a midpoint color value for each of the one or more color arrays for the first reference sample and the one or more color arrays for the second reference sample;
• creating, based on the color values, a reference range for a testable criteria and creating a digital record of the reference range for the testable criteria;
• determining, for a sample, one or more sample color arrays, the one or more sample color arrays having color values corresponding to the one or more liquid blood properties of the sample;
• comparing a sample color array of the one or more sample color arrays with the reference range for the testable criteria;
• determining a presence of one or more defects in the sample when the sample color array is outside of the reference range for the testable criteria;
• and
• upon determining a presence of the one or more defects, communicating a readable error code including an indication of the one or more defects, to an automated method control.