US 10,308,979 B2 · Target enrichment and labeling for multi-kilobase DNA

Abstract

This disclosure provides a method comprising: a) clamping the top and bottom strands of a double stranded DNA molecule to produce a duplex in which the top and bottom strands are linked; b) denaturing the duplex to produce a denatured product; and c) renaturing the denatured product in the presence of a labeled oligonucleotide that is complementary to a sequence of nucleotides in the double stranded DNA molecule, thereby producing a D-loop-containing product. Kits for performing the method and products made by the method are also provided.

Claims17

Dependent claims

A method comprising:

a) clamping the top and bottom strands of a double stranded DNA molecule to produce a duplex in which the top and bottom strands are linked;
b) denaturing the duplex to produce a denatured product;
and
c) renaturing the denatured product in the presence of a labeled oligonucleotide, wherein the labeled oligonucleotide hybridizes to a sequence of nucleotides on one strand in the double stranded DNA molecule, thereby producing a renatured, double-stranded product that comprises a triplex region comprising the labeled oligonucleotide.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

13/830,408Filed 14 Mar 2013 · us provisional application

Priority

1 Jun 2012

Published

US 2013/0323725 A15 Dec 2013

Granted

4 Jun 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification3 codes

C12Q 1/6813
Involving nucleic acids: hybridisation assays
C12Q 1/6806
Involving nucleic acids: preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms

Target enrichment and labeling for multi-kilobase DNA

Life of this patent

Target enrichment and labeling for multi-kilobase DNA

Life of this patent

Abstract

Claims17