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US 10,308,978 B2Granted
Transposon nucleic acids comprising a calibration sequence for DNA sequencing
AssigneeThermo Fisher Scientific Baltics UAB
InventorsIan Kavanagh, Laura-Leena Kiiskinen, Heli Haakana
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Abstract
Transposon nucleic acids comprising a transposon end sequence and a calibration sequence for DNA sequencing in the transposon end sequence. In one embodiment, the transposon end sequence is a Mu transposon end. A method for the generation of DNA fragmentation library based on a transposition reaction in the presence of a transposon end with the calibration sequence providing facilitated downstream handling of the produced DNA fragments, e.g., in the generation of sequencing templates.
Claims19
A method for in vitro assembly of a plurality of transpososome complexes, comprising:
- contacting under a condition for forming a transpososome complex (i) a plurality of MuA transposase enzymes, and (ii) a plurality of modified MuA transposon end sequences each comprising the nucleotide sequence of SEQ ID NO:5, thereby forming a plurality of transpososome complexes.
Dependencies are inferred from the claim text.