Device and method for processing a biological sample and analysis system for analyzing a biological specimen

An analysis system for analyzing a biological sample, the analysis system comprising:

• an apparatus configured to process the biological sample of organic cells, the apparatus including:
• a first substrate having a first cavity forming a first chamber portion of a chamber, the chamber configured to receive the biological sample;
• a second substrate having a second cavity forming a second chamber portion of the chamber;
• a filter element having a plurality of perforations, the filter element arranged between the first cavity and the second cavity and configured to hold back a plurality of organic cells of the biological sample at the plurality of perforations when moving the biological sample from the first cavity to the second cavity through the filter element;
• an electrically conductive structure arranged at the filter element and configured to bring about lysis of the plurality of organic cells as the plurality of organic cells are held back by the filter element and/or to duplicate and/or detect defined sections of exposed DNA;
• a first fluid channel extending between a main side of the first substrate and the first cavity, the main side of the first substrate facing away from the first cavity, and a second fluid channel extending between the main side of the first substrate and the second cavity, wherein one of the first fluid channel and the second fluid channel is configured to admit the biological sample into the chamber and the other of the first fluid channel and the second fluid channel is configured to release the biological sample it from the chamber;
• a detection region configured to receive a lysate obtained during lysis of the organic cells, the detection region arranged adjacent to at least one of the first fluid channel and the second fluid channel at the main side of the first substrate;
• and
• a detection unit configured to detect a predetermined cell constituent of the biological sample extracted from the organic cells in the apparatus, wherein
• the electrically conductive structure comprises a first conductor track between a first connection region of the electrically conductive structure and a second connection region of the electrically conductive structure, the first conductor track configured to apply a first voltage between the first connection region and the second connection region, and
• the electrically conductive structure comprises a second conductor track between a third connection region of the electrically conductive structure and a fourth connection region of the electrically conductive structure, the second conductor track configured to apply a second voltage between the third connection region and the fourth connection region the detection unit fluidically coupled to the apparatus.

A method for processing a biological sample, the method comprising:

• moving the biological sample between a first cavity and a second cavity through a filter element and an electrically conductive structure, the first cavity laid out in a first substrate and forming a first chamber portion of a chamber for receiving the biological sample, the second cavity laid out in a second substrate and forming a second chamber portion of the chamber, the filter element comprising a plurality of perforations embodied between the first cavity and the second cavity in order to hold back a plurality of organic cells of the biological sample at the plurality of perforations, the electrically conductive structure arranged at the filter element;
• and
• applying at least one electric voltage to the electrically conductive structure in the presence of a lysing agent flushed into the chamber by applying a first electric voltage via a first conductor track connecting a first connection region of the electrically conductive structure with a second connection region of the electrically conductive structure, and by applying a second electric voltage via a second conductor track connecting a thud connection region of the electrically conductive structure to a fourth connection region of the electrically conductive structure, thereby lysing the plurality of organic cells held back at the plurality of perforations.