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US 10,308,972 B2Granted
Whole-cell system for cytochrome P450 monooxygenases biocatalysis
AssigneeSanofi Chimie
InventorsAdrian Gerber, Frank Hannemann, Sabrina Bleif, Michael Kleser, Rita Bernhardt
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Abstract
The subject of the present invention is a whole-cell catalysis process for converting substrates of cytochrome P450 monooxygenases of eukaryotic origin into valuable biotechnological products. The subject of the present invention is also microorganisms genetically engineered to achieve those biotransformations with high rates and processes to prepare these microorganism strains.
Claims12
A genetically engineered microorganism capable of converting cholesterol, cholesterol analogs, and derivatives thereof into steroid hormones precursors,
- wherein said microorganism comprises at least one DNA sequence encoding a cytochrome P450 of eukaryotic origin, an exogenous DNA sequence encoding Adx, and an exogenous DNA sequence encoding AdR,
- wherein said microorganism is Bacillus megaterium,
- wherein said cytochrome P450 of eukaryotic origin is selected from the group consisting of CYP11A1, CYP17A1, CYP11B1, CYP11B2, CYP3A4, CYP46A1, CYP27A1, CYP21A1, and CYP21A2, and
- wherein the gene PhaC is overexpressed by introducing into said microorganism an exogenous DNA sequence encoding PhaC.
1 more element
Dependencies are inferred from the claim text.