Microorganism for expressing a human membrane protein
AssigneeNovozymes A/S
InventorsMichael Schilling, Christine Lang, Andreas Raab
Life of this patent
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Abstract
The invention relates to an isolated, genetically modified, living non-mammal organism, having increased HMG-CoA-reductase activity compared to the wild type, and having reduced C24-methyltransferase and/or delta22-desaturase activity compared to the wild type. The invention is characterized in that the organism has increased dehydrocholesterol-delta70-reductase activity compared to the wild type. The invention further relates to different uses of such an organism, to a test kit comprising such an organism, and to a membrane extract of such an organism.
Claims11
A method making a membrane protein of a mammal, the method comprising:
- cultivating an isolated genetically modified living non-mammal yeast organism, having an increased HMG-CoA reductase activity compared to the wild type, and having a reduced C24 methyltransferase and/or delta22 desaturase activity compared to the wild type, wherein the organism has an increased dehydrochole-sterol-delta7 reductase activity compared to the wild type and is additionally transformed with a gene coding for a membrane protein of a mammal;
- and
- isolating the membrane protein from the cultivation supernatant and/or from the yeast.
Dependencies are inferred from the claim text.