Method of preparing biological material

A method of preparing biological material from a biological sample selected from the group consisting of blood and sputum samples, the method including the step of altering at least one constitutive characteristic of the biological sample in the presence of a capturing scaffold by adding a lysis buffer containing a solubilising agent in the form of a chaotropic salt selected from the group consisting of urea, thiourea, guanidine hydrochloride, lithium perchlorate, sodium iodine, sodium perchlorate, guanidine isothiocyanate, guanidine carbonate, guanidine thiocyanate, derivatives of said chaotropic salts and combinations thereof and a detergent to the biological sample, and concomitantly adding purified starch to the biological material together with the lysis buffer for neutralising any PCR inhibitors present in the biological material, and concomitantly physically treating the biological sample through agitation and elevating the temperature of the biological sample above 40 degrees Celsius and up to a 100 degrees Celsius;

• for simultaneously:
• inhibiting coagulation of the biological sample;
• lysing the biological sample to release biological material from the biological sample thus making the biological material available;
• and
• capturing at least one fraction of the biological material on the capturing scaffold in a single chamber or well such that the biological material captured on the capturing scaffold is usable directly for analysis without any purification required and wherein the biological material captured on the capturing scaffold can be subsequently air dried and stored at ambient temperature.