Polymerase variants

An isolated polypeptide having a DNA polymerase activity comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:

• 1, wherein the amino acid sequence having at least 95% sequence identity to SEQ ID NO:
• 1 comprises at least two amino acid substitutions relative to SEQ ID NO:
• 2, wherein one of said at least two amino acid substitutions corresponds to S366 of SEQ ID NO:
• 2 and wherein said amino acid corresponding to position S366 is substituted with A or L.