Omega-transaminase mutants with activity improvements toward ketones and methods for producing optically pure amines
AssigneeIndustry-Academic Cooperation Foundation, Yonsei University
InventorsJong-Shik Shin, Eul-Soo Park, Sang-woo Han
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Abstract
Provided is an omega-transaminase mutant with improved activity using a point mutation in an active site of an omega-transaminase. Specifically, provided is a method for improving activity and extending a substrate spectrum of omega-transaminase by introducing a point mutation into a wild-type omega-transaminase rendered by replacing tryptophan at position 58 with the other amino acid.
Claims8
An isolated omega-transaminase mutant of a wild-type omega-transaminase having the amino acid sequence of SEQ ID NO:
- 1 for producing an optically active amine from a ketone substrate,
- wherein the omega-transaminase mutant has a point mutation at position 58 rendered by replacing tryptophan with a first different amino acid selected from the group consisting of alanine, cysteine, histidine, isoleucine, leucine, methionine, asparagine, glutamine, serine, threonine, valine, and tyrosine;
- and
- wherein the omega-transaminase mutant has up to ten additional point mutations.
An isolated omega-transaminase mutant of a wild-type omega-transaminase having the amino acid sequence of SEQ ID NO:
- 1 for producing an optically active amine from a ketone substrate,
- wherein the omega-transaminase mutant has a point mutation at position 58 rendered by replacing tryptophan with a first different amino acid,
- wherein the omega-transaminase mutant has a second point mutation rendered by replacing at least one amino acid selected from the group consisting of leucine at position 57, valine at position 154, and isoleucine at position 261,
- wherein the isolated omega-transaminase mutant has an amino acid sequence selected from the group consisting of the amino acid sequences of SEQ ID NOS:
- 20 and 27 to 30;
- and
- wherein the omega-transaminase mutant has up to ten additional point mutations.
Dependencies are inferred from the claim text.