Dried spore germinative compound mixtures
AssigneeEnvera LIC, LLC
InventorsTommie Eugene Hashman, Michael Matheny
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Abstract
In one aspect, the present invention is directed to a dried intimate mixture comprising a bacteria spore and a germinative compound, and methods for preparing the intimate mixture. In another aspect, this invention is directed to a composition comprising such an intimate mixture. The invention also relates to methods for increasing the germination, growth, metabolism, and/or enzyme activity of a bacteria spore comprising preparing an intimate mixture of a bacteria spore and a germinative compound.
Claims20
A dried mixture comprising a bacterial spore and an L-amino acid, wherein the dried mixture is prepared by drying a solution comprising the L-amino acid and the bacterial spore, wherein the L-amino acid is adsorbed to or absorbed by the bacterial spore and binds to said bacterial spore's germination initiator sites when the mixture reaches an appropriate environment for germination, and wherein the bacterial spore in the dried mixture germinates more rapidly than a bacterial spore that is not dried with the L-amino acid.
A method for preparing a dried mixture comprising a bacterial spore and an L-amino acid, the method comprising:
- a) preparing a solution comprising a bacterial spore and an L-amino acid;
- and
- b) drying the solution to obtain a dried mixture comprising the bacterial spore and the L-amino acid,
- wherein the L-amino acid is adsorbed to or absorbed by the bacterial spore and binds to said bacterial spore's germination initiator sites when the mixture reaches an appropriate environment for germination, and wherein the bacterial spore in the dried mixture germinates more rapidly than a bacterial spore that is not dried with the L-amino acid.
A method for increasing the germination of bacterial spores, comprising:
- a) preparing a solution comprising a bacterial spore and an L-amino acid;
- and
- b) drying the solution to obtain a dried mixture comprising the bacterial spore and the L-amino acid, wherein the L-amino acid is adsorbed to or absorbed by the bacterial spore and binds to said bacterial spore's germination initiator sites when the mixture reaches an appropriate environment for germination;
- and
- c) exposing the mixture to an environment conducive to germination of the bacterial spores, wherein the germination of the bacterial spores in the dried mixture is increased relative to a corresponding bacterial spore formulation that lacks the L-amino acid.
Dependencies are inferred from the claim text.