Treatment of cancer using humanized anti-EGFRvIII chimeric antigen receptor

A humanized anti-EGFRvIII binding domain comprising:

• (a) a heavy chain immunoglobulin variable region comprising:
• (i) a CDR1 comprising the sequence DYYIH (SEQ ID NO:
• 22);
• (ii) a CDR2 comprising the sequence RIDPENDETKYGPIFQG (SEQ ID NO:
• 23);
• and
• (iii) a CDR3 comprising the sequence RGGVY (SEQ ID NO:
• 24);
• and
• (b) a light chain immunoglobulin variable region comprising:
• (i) a CDR1 comprising the sequence KSSQSLLDSDGKTYLN (SEQ ID NO:
• 26);
• (ii) a CDR2 comprising the sequence LVSKLDS (SEQ ID NO:
• 27);
• and
• (iii) a CDR3 comprising the sequence WQGTHFPGT (SEQ ID NO:
• 28).