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AssigneeFraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e. V.
InventorsMarlitt Stech, Katja Hanack, Katrin Messerschmidt, Stefan Kubick
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The invention relates to a method for producing polyclonal antibodies against an antigenic target protein which comprises inducing an immune response in a host by applying an immunogenic composition comprising membrane vesicles incorporating said antigenic target protein to said host and obtaining antibodies against said target protein from the host's serum. A more specific embodiment of this method comprises at least the following steps: a) synthesizing the target protein my means of an in vitro translation reaction in a reaction medium comprising a nucleic acid template coding for the target protein and a cell lysate which contains membrane vesicles; b) separating membrane vesicles comprising the synthesized target protein from the medium; c) providing the membrane vesicles comprising the synthesized target protein in a physiologically compatible medium; d) applying the membrane vesicles of step c) to a host; e) testing a serum sample of the host for a specific immune response against said target protein; and f) obtaining specific antibodies against said target protein from the serum of a host exhibiting a specific immune response against said target protein.
A method for producing polyclonal antibodies against an antigenic target protein which comprises inducing an immune response in a host by applying an immunogenic composition comprising membrane vesicles incorporating said antigenic target protein to said host and obtaining antibodies against said target protein from a serum of the host, wherein the membrane vesicles are ER-derived and the antigenic target protein is de novo synthesized in a eukaryotic cell-free system.
A method for accumulating a target protein in vesicles of a membrane vesicle preparation, comprising at least the following steps:
Dependencies are inferred from the claim text.