Anti-GP73 monoclonal antibodies and methods of obtaining the same

A method for determining GP73 concentration in a test sample, the method comprising:

• (a) contacting the test sample with at least one capture antibody comprising (i) a variable heavy chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO:
• 18, a CDR2 comprising the amino acid sequence of SEQ ID NO:
• 19, and a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 20, and (ii) a variable light chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO:
• 22, a CDR2 comprising the amino acid sequence of SEQ ID NO:
• 23, and a CDR3 comprising the amino acid sequence of SEQ ID NO:
• 24, which binds to an epitope on GP73 comprising the amino acid sequence of SEQ ID NO:
• 102 to form a capture antibody-GP73 antigen complex;
• (b) contacting the capture antibody-GP73 antigen complex with at least one detection antibody comprising a detectable label, wherein the detection antibody comprises (i) a variable heavy chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequence of SEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ ID NO:28, and (ii) a variable light chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequence of SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ ID NO:32 which binds to an epitope on GP73 that is not bound by the capture antibody and forms a capture antibody-GP73 antigen-detection antibody complex;
• and
• (c) determining the GP73 concentration in the test sample based on the signal generated by the detectable label in the capture antibody-GP73 antigen-detection antibody complex formed in (b).