Mutated immunoglobulin-binding polypeptides
AssigneeGE Healthcare BioProcess R&D AB
InventorsGustav Rodrigo, Mats Ander, Göran Baurén, Tomas Björkman
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Abstract
The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, comprising at least the mutation wherein the glutamine residue at position 9 has been mutated to a tryptophan, leucine, glutamic acid, valine or lysine. The invention also discloses multimers of the polypeptide, as well as separation matrices comprising the multimers or polypeptides.
Claims27
An Fc-binding polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a parental polypeptide, as defined by, or having at least 90% identity to, SEQ ID NO 28, SEQ ID NO 29 or SEQ ID NO 30, comprising at least the mutation wherein the glutamine residue at position 3 has been mutated to leucine, glutamic acid, valine or lysine.
A multimer with improved alkaline stability comprising or consisting essentially of a plurality of polypeptide units, which polypeptide comprises a mutant of a parental polypeptide, as defined by, or having at least 90% identity to, SEQ ID NO 28, SEQ ID NO 29 or SEQ ID NO 30, comprising at least the mutation wherein the glutamine residue at position 3 has been mutated to tryptophan, leucine, glutamic acid, valine or lysine.
An Fc-binding polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as defined by, or having at least 90% identity to, SEQ ID NO 1 or SEQ ID NO 2, of Protein Z, as defined by, or having at least 90% identity to, SEQ ID NO 3, or of a Protein Z variant as defined by, or having at least 90% identity to, SEQ ID NO 4, comprising at least the mutation wherein the glutamine residue at position 9 has been mutated to leucine, glutamic acid, valine or lysine.
A multimer comprising or consisting essentially of a plurality of polypeptide units, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as defined by, or having at least 90% identity to, SEQ ID NO 1 or SEQ ID NO 2, of Protein Z, as defined by, or having at least 90% identity to, SEQ ID NO 3, or of a Protein Z variant as defined by, or having at least 90% identity to, SEQ ID NO 4, comprising at least the mutation wherein the glutamine residue at position 9 has been mutated to tryptophan, leucine, glutamic acid, valine or lysin.
A polypeptide, comprising or consisting essentially of an amino acid sequence selected from the group consisting of:
- SEQ ID NO 31, SEQ ID NO 32, SEQ ID NO 33, SEQ ID NO 34 and SEQ ID NO 35.
Dependencies are inferred from the claim text.