Cell penetrating stapled peptide, manufacturing method therefor, and use thereof
AssigneeSeoul National University R&Db Foundation
InventorsJaehoon Yu, Soonsil Hyun, Yan Lee, Seonju Lee
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Abstract
The present invention relates to a stapled peptide, a preparation method thereof and the use thereof, and more specifically to an amphipathic alpha-helical stapled peptide comprising hydrophobic amino acids and hydrophilic amino acids, a preparation method thereof, and the use thereof for intracellular delivery of an active substance.
Claims6
A method for intracellular delivery of a biologically active substance, which comprises delivering a composition comprising an amphipathic alpha-helical stapled peptide comprising hydrophobic amino acids and hydrophilic amino acids, wherein the stapled peptide comprises any one of the following sequences:
- (SEQ ID NO:
- 1)
- X1KKLLKLX2KKLLKLAG,
- (SEQ ID NO:
- 2)
- LKKX1LKLLKKX2LKLAG,
- (SEQ ID NO:
- 3)
- LKKLX1KLLKKLX2KLAG,
- (SEQ ID NO:
- 4)
- LKKLLKX1LKKLLKX2AG,
- (SEQ ID NO:
- 5)
- LKKLLKLX1KKLLKLX2G,
- (SEQ ID NO:
- 6)
- LKKLLKLLX1KLLKLAX2,
- (SEQ ID NO:
- 7)
- LKHLLHLX1KHLLKLX2G,
- and
- (SEQ ID NO:
- 8)
- CLKKLLKLX1KKLLKLX2G,
- wherein X1 is (R)-2-(7′-octenyl) alanine, and X2 is (S)-2-(4′-pentenyl) alanine.
An amphipathic alpha-helical stapled peptide comprising any one of the following sequences:
- (SEQ ID NO:
- 1)
- X1KKLLKLX2KKLLKLAG,
- (SEQ ID NO:
- 2)
- LKKX1LKLLKKX2LKLAG,
- (SEQ ID NO:
- 3)
- LKKLX1KLLKKLX2KLAG
- (SEQ ID NO:
- 4)
- LKKLLKX1LKKLLKX2AG,
- (SEQ ID NO:
- 5)
- LKKLLKLX1KKLLKLX2G,
- (SEQ ID NO:
- 6)
- LKKLLKLLX1KLLKLAX2,
- (SEQ ID NO:
- 7)
- LKHLLHLX1KHLLKLX2G,
- and
- (SEQ ID NO:
- 8)
- CLKKLLKLX1KKLLKLX2G,
- wherein X1 is (R)-2-(7′-octenyl) alanine, and X2 is (S)-2-(4′-pentenyl) alanine.
Dependencies are inferred from the claim text.