Biomimetic hydrogel scaffolds and related methods
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorShilpa Sant
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Abstract
Provided herein are methods of making a biomimetic hydrogel scaffold comprising a polycation and a polyanion. Also provided are anisotropic biomimetic hydrogel scaffold compositions suitable for use in tissue growth, including bone, muscle, and nerve growth an optionally comprising a carbon allotrope such as graphene. Also provided are methods of producing tissue comprising growing tissue on the biomimetic hydrogel scaffold comprising a polycation and a polyanion.
Claims24
A method of producing a biotherapeutic composition, comprising feeding a first component comprising a positively-charged polyelectrolyte, and a second component comprising a negatively-charged polyelectrolyte through a fluid flow passage to produce a product comprising an anisotropic structure,
- wherein the method further comprises feeding a therapeutic agent into the passage with the first and second components, wherein the therapeutic agent is one or more of:
- basic fibroblast growth factor, acidic fibroblast growth factor, vascular endothelial growth factor, hepatocyte growth factor, insulin-like growth factors 1 and 2, platelet derived growth factor, stromal derived factor 1 alpha, nerve growth factor, ciliary neurotrophic factor, neurotrophin-3, neurotrophin-4, neurotrophin-5, pleiotrophin protein, midkine protein, brain-derived neurotrophic factor, tumor angiogenesis factor, corticotrophin releasing factor, transforming growth factors α and β, granulocyte-macrophage colony stimulating factor, osteopontin, osteocalcin, an interleukin, and/or an interferon, and
- wherein the positively-charged polyelectrolyte has a net positive zeta potential, and the negatively-charged polyelectrolyte has a net negative zeta potential.
A composition comprising an anisotropic assembly of a positively-charged polyelectrolyte and a negatively-charged polyelectrolyte,
- wherein the composition is complexed with a therapeutic agent, and wherein the therapeutic agent is one or more of:
- basic fibroblast growth factor, acidic fibroblast growth factor, vascular endothelial growth factor, hepatocyte growth factor, insulin-like growth factors 1 and 2, platelet derived growth factor, stromal derived factor 1 alpha, nerve growth factor, ciliary neurotrophic factor, neurotrophin-3, neurotrophin-4, neurotrophin-5, pleiotrophin protein, midkine protein, brain-derived neurotrophic factor, tumor angiogenesis factor, corticotrophin releasing factor, transforming growth factors α and β, granulocyte-macrophage colony stimulating factor, osteopontin, osteocalcin, an interleukin, and/or an interferon, and
- wherein the positively-charged polyelectrolyte has a net positive zeta potential, and the negatively-charged polyelectrolyte has a net negative zeta potential.
Dependencies are inferred from the claim text.