Epimorphic regeneration and related hydrogel delivery systems

A method of modulating proline hydroxylase activity, said method comprising:

• providing a composition comprising a prodrug of 1,4-dihydrophenonthrolin-4-one-3-carboxylic acid (1,4-DPCA), said prodrug comprising a 1,4-DPCA component coupled to a cleavable polymer component, and a carrier component comprising a hydrogel comprising a chemical ligation product of
• and wherein each of R1 and R2 is independently selected from hexaglycolic and tripentaerythritolic moieties, each of n1 and n2 is an integer independently selected from 1 to about 201, and Cys is an N-terminal cysteine residue;
• and
• contacting said 1,4-DPCA component of said prodrug with a cellular medium expressing a proline hydroxylase, said cellular medium within a non-regenerative mammal presenting a tissue injury, said 1,4-DPCA component in an amount sufficient to modulate proline hydroxylase activity in said cellular medium and thereby induce a regenerative response.