US 10,307,406 B2 · Methods and compositions for re-activating Epstein-Barr virus and screening compounds therefor

Abstract

In an effort to discover therapies for treating diseases caused by EBV, a novel screening assay for identifying compounds that reactivate EBV latent infection and a family of small molecules based on a tetrahydrocarboline backbone were discovered. Specifically, the compounds have the structure of the formula (I), wherein R1-R11 are defined herein and activate/reactivate EBV in a variety of cell types in a patient and are, therefore, useful in preventing or treating EBV-positive cancer, optionally with an anti-viral agent. In screening these compounds, novel compositions, EBV-positive cell lines, and methods are provided. (Formula I)

Claims12

Dependent claims

A method for reactivating latent Epstein-Barr virus (EBV) in a cell, said method comprising administering a compound of the formula to a subject in need thereof:

wherein:
R1 is H, C1 to C6 alkyl, C1 to C6 alkoxy, or halogen;
R2 is H, C1 to C6 alkyl, or C1 to C6 alkoxy;
R3 is a phenyl group substituted on the C in its second position with —C(O)OCH3;
R4 is H or optionally substituted C1 to C6 alkyl;
R5 to R11 are, independently, selected from the group consisting of H and C1 to C6 alkyl;
and
R13 is H, optionally substituted aryl, or optionally substituted heteroaryl;
or a pharmaceutically acceptable acid salt or base salt or prodrug thereof.

6 more elements

Dependencies are inferred from the claim text.

Facts

Application

14/912,949Filed 29 Aug 2014 · us provisional application

Priority

31 Aug 2013

Published

US 2016/0206601 A121 Jul 2016

Granted

4 Jun 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

A61K 31/437
Ortho- or peri-condensed with heterocyclic ring systems: the heterocyclic ring system containing a five-membered ring having nitrogen as a ring…
A61K 31/19
Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids: carboxylic acids, e.g. valproic acid
A61K 31/522
Purines, e.g. adenine: having oxo groups directly attached to the heterocyclic ring, e.g. hypoxanthine, guanine, acyclovir
A61K 31/69
Medicinal preparations containing organic active ingredients: boron compounds

Methods and compositions for re-activating Epstein-Barr virus and screening compounds therefor

Life of this patent

Methods and compositions for re-activating Epstein-Barr virus and screening compounds therefor

Life of this patent

Abstract

Claims12