Oil-in-water emulsions that contain nucleic acids
AssigneeGlaxosmithkline Biologicals SA
InventorsLuis Brito, Michelle C. Archer, Andrew Geall, Derek O'Hagan, Manmohan Singh
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Abstract
This invention generally relates to cationic oil-in-water emulsions that can be used to deliver nucleic acid molecules, such as an RNA molecule. The emulsion particles comprise an oil core and a cationic lipid. The emulsion particles have an average diameter of about 80 nm to about 180 nm, and the emulsion have an N/P ratio of at least 1.1:1.
Claims27
A method for generating an immune response in a subject, comprising administering to the subject an effective amount of an immunogenic cationic oil-in-water emulsion comprising emulsion particles that contain an oil core and a cationic lipid, and a nucleic acid molecule that is complexed to the emulsion particles, wherein:
- a) the nucleic acid is RNA that encodes a polypeptide antigen,
- b) the average diameter of the emulsion particles is from about 80 nm to about 180 nm, and
- c) the N/P of the emulsion is at least 4:1;
- with the proviso that the nucleic acid molecule does not encode secreted alkaline phosphatase, and the further proviso that the nucleic acid molecule is not the RNA encoded by the plasmid A317, the sequence of which is given by SEQ ID NO:1;
- whereby the immune response is generated in the subject.
Dependencies are inferred from the claim text.