Yield QTLs in cucumber plants
AssigneeNunhems B.V.
InventorsGerhard T. M. Reuling, Peter Arnold Gijsbert Kraan, Frank Beenders, Marion Van De Wal, Freddy Hermans, Hans-Peter Koelewijn, Steven D. Tanksley, Alexandra M. Casa
Life of this patent
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Abstract
The present invention relates to cultivated cucumber plants comprising a yield QTL on chromosome 2 of their genome.
Claims32
A cultivated Cucumis sativus var. sativus plant, or part thereof, comprising an introgression fragment on chromosome 2 in homozygous or heterozygous form, wherein said introgression fragment is the fragment as found on chromosome 2 in seeds of which a representative sample has been deposited under accession number NCUMB 42262, or a smaller fragment derived therefrom, and confers an increase in cucumber fruit yield, and wherein said introgression fragment or smaller fragment comprises at least 1 of the following markers:
- a) the AG or GG genotype for the Single Nucleotide Polymorphism marker SNP 01 in SEQ ID NO:
- 1;
- b) the AG or GG genotype for the Single Nucleotide Polymorphism marker SNP 02 in SEQ ID NO:
- 2;
- c) the AG or GG genotype for the Single Nucleotide Polymorphism marker SNP 03 in SEQ ID NO:
- 3;
- d) the GT or GG genotype for the Single Nucleotide Polymorphism marker SNP 04 in SEQ ID NO:
- 4;
- e) the AC or CC genotype for the Single Nucleotide Polymorphism marker SNP 05 in SEQ ID NO:
- 5;
- f) the CT or TT genotype for the Single Nucleotide Polymorphism marker SNP 06 in SEQ ID NO:
- 6;
- g) the AG or GG genotype for the Single Nucleotide Polymorphism marker SNP 07 in SEQ ID NO:
- 7;
- h) the CT or TT genotype for the Single Nucleotide Polymorphism marker SNP 08 in SEQ ID NO:
- 8,
- i) the CT or CC genotype for the Single Nucleotide Polymorphism marker SNP 09 in SEQ ID NO:
- 9,
- j) the GT or GG genotype for the Single Nucleotide Polymorphism marker SNP 10 in SEQ ID NO:
- 10;
- and/or
- k) the AG or AA genotype for the Single Nucleotide Polymorphism marker SNP 11 in SEQ ID NO:
- 11.
A method for identifying a cultivated C. sativus var. sativus plant comprising an introgression fragment on chromosome 2, wherein said introgression fragment is as found in NCIMB 42262, or a smaller fragment derived therefrom, comprising:
- a) screening a population of cultivated C. sativus var. sativus plants using a molecular marker assay which detects at least one SNP marker of:
- SNP_01 to SNP_11 for detecting the introgression fragment on chromosome 2;
- and
- b) identifying and/or selecting a plant comprising:
- i) at least 1 of the SNP markers of SNP_01 to SNP_11 for detecting the introgression fragment on chromosome 2;
- or
- ii) at least 2 consecutive markers from SNP_01 to SNP_11 for detecting the introgression fragment on chromosome 2;
- or
- iii) at least 1 marker[s] from SNP_2, SNP_5, SNP_7, SNP_9 and/or SNP_10 for detecting the introgression fragment on chromosome 2;
- or
- iv) at least marker SNP_06 and optionally also marker SNP_05 and/or SNP_07 for detecting the introgression fragment on chromosome 2.
A method of producing C. sativus F1 hybrid plants comprising an introgression fragment conferring enhanced fruit yield comprising:
- a) crossing a first inbred cucumber plant comprising a recombinant chromosome 2 in homozygous form having an introgression fragment comprising an allele conferring enhanced yield, wherein said introgression fragment is as in NCIMB 42262 with a second inbred cucumber plant, and
- b) collecting F1 hybrid seeds from said cross.
A method for generating progeny of NCIMB 42262, said method comprising:
- a) selfing a plant grown from seeds deposited under accession number NCIMB 42262 one or more times or crossing said plant one or more times with another cucumber plant to generate progeny seeds;
- b) screening said progeny seeds or plants grown from said progeny seeds or parts of the progeny seeds or plants using a molecular marker assay which detects at least one of:
- SNP_01 to SNP_11 for detecting the introgression fragment on chromosome 2, and
- c) identifying and/or selecting a progeny plant comprising:
- i) at least 1 of the SNP markers of SNP_01 to SNP_11 for detecting the introgression fragment on chromosome 2;
- or
- ii) at least 2 consecutive markers from SNP_1 to SNP_11 for detecting the introgression fragment on chromosome 2;
- or
- iii) at least 1 marker[s] from SNP_2, SNP_5, SNP_7, SNP_9 and SNP10 for detecting the introgression fragment on chromosome 2;
- or
- iv) at least marker SNP_06 and optionally also marker SNP_05 and/or SNP_07 for detecting the introgression fragment on chromosome 2.
Dependencies are inferred from the claim text.