Yield QTLs in cucumber plants
AssigneeNunhems B.V.
InventorsGerhard T. M. Reuling, Peter Arnold Gijsbert Kraan, Frank Beenders, Marion Van De Wal, Freddy Hermans, Hans-Peter Koelewijn, Steven D. Tanksley, Alexandra M. Casa
Life of this patent
Estimating the term…
Abstract
The present invention relates to cultivated cucumber plants comprising a yield QTL on chromosome 2 and/or chromosome 6 of their genome.
Claims27
A Cucumis sativus var. sativus plant, or part thereof, comprising an introgression fragment on chromosome 6 in homozygous or heterozygous form, wherein said introgression fragment is the fragment as found on chromosome 6 in seeds of which a representative sample has been deposited under accession number NCIB 42262, or a smaller fragment derived therefrom, and confers an increase in cucumber fruit yield, and wherein said introgression fragment or smaller fragment comprises at least 1 of the following markers:
- a) the AG or AA genotype for the Single Nucleotide Polymorphism marker SNP 12 in SEQ ID NO:
- 12;
- b) the AG or AA genotype for the Single Nucleotide Polymorphism marker SNP 13 in SEQ ID NO:
- 13;
- c) the AG or GG genotype for the Single Nucleotide Polymorphism marker SNP 14 in SEQ ID NO:
- 14;
- d) the CT or TT genotype for the Single Nucleotide Polymorphism marker SNP 15 in SEQ ID NO:
- 15;
- e) the AG or AA genotype for the Single Nucleotide Polymorphism marker SNP 16 in SEQ ID NO:
- 16;
- f) the CT or TT genotype for the Single Nucleotide Polymorphism marker SNP 17 in SEQ ID NO:
- 17;
- g) the CT or CC genotype for the Single Nucleotide Polymorphism marker SNP 18 in SEQ ID NO:
- 18;
- h) the AC or AA genotype for the Single Nucleotide Polymorphism marker SNP 19 in SEQ ID NO:
- 19;
- i) the AC or AA genotype for the Single Nucleotide Polymorphism marker SNP 20 in SEQ ID NO:
- 20;
- j) the AG or GG genotype for the Single Nucleotide Polymorphism marker SNP 21 in SEQ ID NO:
- 21;
- k) the CT or CC genotype for the Single Nucleotide Polymorphism marker SNP 22 in SEQ ID NO:
- 22;
- I) the AG or AA genotype for the Single Nucleotide Polymorphism marker SNP 23 in SEQ ID NO:
- 23;
- m) the CT or CC genotype for the Single Nucleotide Polymorphism marker SNP 24 in SEQ ID NO:
- 24;
- n) the AG or GG genotype for the Single Nucleotide Polymorphism marker SNP 25 in SEQ ID NO:
- 25;
- o) the CT or CC genotype for the Single Nucleotide Polymorphism marker SNP 26 in SEQ ID NO:
- 26;
- p) the AG or AA genotype for the Single Nucleotide Polymorphism marker SNP 27 in SEQ ID NO:
- 27;
- q) the CT or CC genotype for the Single Nucleotide Polymorphism marker SNP 28 in SEQ ID NO:
- 28;
- r) the CT or CC genotype for the Single Nucleotide Polymorphism marker SNP 29 in SEQ ID NO:
- 29;
- or
- s) the GT or TT genotype for the Single Nucleotide Polymorphism marker SNP 30 in SEQ ID NO:
- 30.
A method for identifying a cultivated C. sativus var. sativus plant comprising an introgression fragment on chromosome 6, wherein said introgression fragment is as found in NCIMB 42262, or a smaller fragment derived therefrom, comprising:
- a) screening a population of cultivated C. sativus var. sativus plants using a molecular marker assay which detects at least one SNP marker of:
- SNP_12 to SNP_30 for detecting the introgression fragment on chromosome 6;
- and
- b) identifying and/or selecting a plant comprising:
- i) at least 1 of the SNP markers of SNP_12 to SNP_30 for detecting the introgression fragment on chromosome 6;
- or
- ii) at least 2 consecutive markers from SNP_12 to SNP_30 for detecting the introgression fragment on chromosome 6;
- or
- iii) at least 1 marker from SNP_12, SNP_13, SNP_18 to SNP_26, SNP_28 and/or SNP_30 for detecting the introgression fragment on chromosome 6;
- or
- iv) at least 1, 2 or 3 markers selected from SNP_13, SNP_18 and SNP_28 for detecting the introgression fragment on chromosome 6.
A method of producing C. sativus F1 hybrid plants comprising an introgression fragment conferring enhanced fruit yield comprising:
- a) crossing a first inbred cucumber plant comprising a recombinant chromosome 6 in homozygous form having an introgression fragment comprising an allele conferring enhanced yield, wherein said introgression fragment is as in NCIMB 42262 with a second inbred cucumber plant, and
- b) collecting F1 hybrid seeds from said cross.
A method for generating progeny of NCIMB 42262, said method comprising:
- a) selfing a plant grown from seeds deposited under accession number NCIMB 42262 one or more times or crossing said plant one or more times with another cucumber plant to generate progeny seeds;
- b) screening said progeny seeds or plants grown from said progeny seeds or parts of the progeny seeds or plants using a molecular marker assay which detects at least one of:
- SNP_12 to SNP_30 for detecting the introgression fragment on chromosome 6, and
- c) identifying and/or selecting a progeny plant comprising:
- i) at least 1 of the SNP markers of SNP_12 to SNP_30 for detecting the introgression fragment on chromosome 6;
- or
- ii) at least 2 consecutive markers selected from SNP_12 to SNP_30 for detecting the introgression fragment on chromosome 6;
- or
- iii) at least 1 marker from SNP_12, SNP_13, SNP_18 to SNP_26, SNP_28 and SNP_30 for detecting the introgression fragment on chromosome 6;
- or
- iv) at least 1 marker selected from SNP_13, SNP_18 and SNP_28 for detecting the introgression fragment on chromosome 6.
Dependencies are inferred from the claim text.