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US 10,246,741 B2Granted
Coupling method
AssigneeOxford Nanopore Technologies PLC
InventorsJames Clarke, James White, John Milton, Clive Brown
Life of this patent
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Abstract
The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
Claims29
A method for detecting polynucleotides, comprising:
- (a) providing a membrane in which is present a nanopore that provides a channel through the membrane;
- (b) contacting the membrane, in an ionic solution, with polynucleotides, wherein following contact with the membrane the polynucleotides are tethered to the membrane via a tethering group;
- (c) applying a potential difference across the membrane and detecting a first polynucleotide using the nanopore, from among the polynucleotides tethered to the membrane;
- and
- (d) detecting a second polynucleotide, from among the polynucleotides tethered to the membrane, using the same nanopore, wherein the second polynucleotide is not the first polynucleotide.
2 more elements
Dependencies are inferred from the claim text.