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US 10,246,736 B2Granted
Methods and apparatuses for nucleic acid shearing by sonication
AssigneeLife Technologies Corporation
InventorsVladimir I. Bashkirov, Umberto Ulmanella, Robert G. Eason, Bradford J. Taft
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Abstract
Methods and kits for preparing nucleic acid fragments from a sample of purified nucleic acid are provided. Alternatively, chromatin or other long polymers can be sheared with similar methods and kits.
Claims11
A method for preparing a gene fragment library, the method comprising:
- a) providing a suspension comprising a sample of purified genomic nucleic acid and monodisperse particles, wherein the suspension is an aqueous solution without nonaqueous solvent:
- b) sonicating the suspension, thereby generating nucleic acid fragments:
- c) collecting the nucleic acid fragments;
- and
- d) preparing a gene fragment library by ligating the nucleic acid fragments to nucleic acid adaptors.
2 more elements
Dependencies are inferred from the claim text.