Methods for preparing samples for nucleic acid amplification
AssigneeRoche Molecular Systems, Inc.
InventorsClint Pereira, Cathal Joseph McElgunn, Laurence Carlo Tisi
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Abstract
The present invention is in the field of sample preparation. In particular, it relates to methods for preparing samples prior to performing nucleic acid amplification.
Claims18
A method for passing a liquid sample through a porous solid matrix, comprising the steps of sealing the liquid sample within a container which comprises a porous solid matrix as at least a part of the container and raising a temperature to increase pressure inside the container, thereby to cause the liquid sample to pass through the porous solid matrix, wherein the liquid sample includes a biological sample and a buffer.
A method for purifying nucleic acids from a liquid sample which comprises nucleic acids and inhibitors of nucleic acid amplification, wherein the method comprises the steps of
- (a) contacting the liquid sample with a porous solid matrix which binds inhibitors of nucleic acid amplification more strongly than nucleic acids, wherein heat is applied to the porous solid matrix and the liquid sample;
- and
- (b) separating the liquid sample comprising unbound nucleic acids from the porous solid matrix, wherein
- the liquid sample is passed through the porous solid matrix by sealing the liquid sample within a container comprising the porous solid matrix and the heat that is applied raises a temperature to increase pressure inside the container, thereby to cause the liquid to pass through the porous solid matrix, and
- the liquid sample includes a buffer.
Dependencies are inferred from the claim text.