System and method for detecting alpha-methylacyl-CoA racemase (AMACR) and prostate cancer
AssigneeCase Western Reserve University
InventorsChung-Chiun Liu, Laurie Dudik, Po-Yuam Lin
Life of this patent
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Abstract
A detection system for determining alpha-methylacyl-CoA (AMACR) levels in a bodily sample includes at least one reaction solution for generating H2O2 upon combination with AMACR in the bodily sample and a biosensor for determining a level of generated H2O2. The reaction solution includes a (2R)-2-methylacyl-CoA epimer that can be chirally inverted by AMACR to a (2S)-2-methylacyl-CoA epimer and an enzyme that carries out beta oxidation with the (2S)-2-methylacyl-CoA epimer to generate hydrogen peroxide (H2O2).
Claims19
A detection system device for determining alpha-methylacyl-CoA (AMACR) levels in a bodily sample, comprising:
- at least one reaction solution for generating H2O2 upon combination with AMACR in the bodily sample, the reaction solution including a (2R)-2-methylacyl-CoA epimer that can be chirally inverted by AMACR to a (2S)-2-methylacyl-CoA epimer and peroxisomalacyl-coenzyme A oxidase 3 (ACOX3) that carries out beta oxidation with the (2S)-2-methylacyl-CoA epimer to generate hydrogen peroxide (H2O2);
- and a biosensor for determining the level of generated H2O2.
An assay device for detecting prostate cancer in a subject comprising:
- at least one reaction solution for generating H2O2 upon combination with AMACR in a bodily sample obtained from a subject, the reaction solution including a (2R)-2-methylacyl-CoA epimer that can be chirally inverted by AMACR to a (2S)-2-methylacylCoA epimer and peroxisomalacyl-coenzyme A oxidase 3 (ACOX3) that carries out beta oxidation with the (2S)-2-methylacyl-CoA epimer to generate hydrogen peroxide (H2O2);
- and a biosensor for determining the level of generated H2O2.
Dependencies are inferred from the claim text.