Variant thioesterases and methods of use
AssigneeCorbion Biotech, Inc.
InventorsDavid Davis, Scott Franklin, Jeffrey L. Moseley, Riyaz Bhat
Life of this patent
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Abstract
The present invention relates to variant thioesterases and their use in plants, e.g., to increase enzymatic activity and to promote increased production of mid-chain length fatty acids (e.g., 8 to 14 carbons) and at desired ratios. Further disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods described herein.
Claims14
A non-natural fatty acyl-ACP thioesterase wherein the non-natural thioesterase has at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to SEQ ID NO:
- 1 and comprises:
- i) Tyrosine (Y) or phenylalanine (F) at the position corresponding to position 163 of SEQ ID NO:
- 1 and lysine (K), or alanine (A) at the position corresponding to position 186 of SEQ ID NO:
- 1;
- or
- ii) Phenylalanine (F) at the position corresponding to position 163 of SEQ ID NO:
- 1 and/or proline (P), lysine (K), or alanine (A) at the position corresponding to position 186 of SEQ ID NO:
- 1, wherein the non-natural thioesterase catalyzes the production of increased levels of C8:0 and C10:0 fatty acids in comparison to a wild-type thioesterase.
An isolated polynucleotide encoding a non-natural fatty acyl-ACP thioesterase wherein the non-natural thioesterase has at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to SEQ ID NO:
- 1 and comprises:
- i) Tyrosine (Y) or phenylalanine (F) at the position corresponding to position 163 of SEQ ID NO:
- 1 and lysine (K), or alanine (A) at the position corresponding to position 186 of SEQ ID NO:
- 1;
- or
- ii) Phenylalanine (F) at the position corresponding to position 163 of SEQ ID NO:
- 1 and/or proline (P), lysine (K), or alanine (A) at the position corresponding to position 186 of SEQ ID NO:
- 1, wherein the non-natural thioesterase catalyzes the production of increased levels of C8:0 and C10:0 fatty acids in comparison to a wild-type thioesterase.
Dependencies are inferred from the claim text.