Double strand RNA as molecular biopesticides for RNA interference through feeding in the hemipteran invasive insect pest, brown marmorated stink bug
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsSaikat Kumar B. Ghosh, Dawn E. Gundersen-Rindal
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Abstract
The present disclosure provides compositions and methods of delivering double strand ribonucleic acid (dsRNA) to control the insect pest, the brown marmorated stink bug. Several dsRNA species that target genes are disclosed herein that can be used individually or in combination. The dsRNA species can be used to induce RNA-mediated interference (RNAi) to decrease target gene expression. RNAi can be used alone, or in combination with other pest control measures to target the brown marmorated stink bug.
Claims13
A double-stranded ribonucleic acid (dsRNA) comprising a sense region with at least 95% sequence identity to any one of SEQ ID NOs:
- 56, 66, 67, or 68 and an antisense region comprising a second sequence complementary to the sense region, wherein said dsRNA is capable of inducing ribonucleic acid interference (RNAi) in an insect.
A method of controlling H. halys comprising the steps of:
- a) providing a living plant material containing at least one double-strand RNA (dsRNA) wherein the at least one dsRNA molecule comprises a sense region with at least 95% sequence identity to SEQ ID NOs:
- 56, 66, 67, or 68 and an antisense region comprising a second sequence complementary to the sense region, wherein the at least one dsRNA molecule is distributed throughout at least part of the living plant material's vascular tissues and wherein the living plant material does not contain genetic information allowing for the production of the at least one double strand dsRNA molecule;
- b) allowing the insect to ingest a sufficient amount of the at least one dsRNA molecule, by feeding on the plant material, to interfere with the production of at least one protein targeted by the at least one dsRNA molecule, thereby inducing RNAi in the insect, and;
- c) controlling the insect via RNAi.
Dependencies are inferred from the claim text.