Back to results
US 10,246,705 B2Granted
Linking sequence reads using paired code tags
AssigneeIlumina, Inc.
InventorsFrank J. Steemers, Kevin L. Gunderson, Thomas Royce, Natasha Pignatelli, Igor Goryshin, Nicholas Caruccio
Life of this patent
Estimating the term…
Abstract
Artificial transposon sequences having code tags and target nucleic acids containing such sequences. Methods for making artificial transposons and for using their properties to analyze target nucleic acids.
Claims10
An artificial transposon, comprising:
- a first transposase recognition site,
- a second transposase recognition site,
- a barcode disposed therebetween, wherein the barcode comprises a double-stranded nucleic acid sequence comprising
- a first strand barcode and
- a second strand barcode, wherein the first strand barcode and the second strand barcode comprise non-complementary sequences;
- and
- a fragmentation site disposed within the first strand barcode and the second strand barcode.
4 more elements
A DNA library preparation kit, comprising:
- a plurality of artificial transposons, wherein each artificial transposon of the plurality of artificial transposon comprises:
- a first transposase recognition site;
- a second transposase recognition site;
- a barcode disposed therebetween, wherein the barcode comprises a double-stranded nucleic acid sequence comprising
- a first strand barcode and
- a second strand barcode, wherein the first strand barcode and the second strand barcode comprise non-complementary sequences;
- and
- a fragmentation site disposed within the first strand barcode and the second strand barcode;
- and
- wherein each barcode of each artificial transposon of the plurality of artificial transposons is distinguishable from one another.
7 more elements
Dependencies are inferred from the claim text.