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AssigneesCentre National de la Recherche Scientifique (CNRS), Universite La Rochelle
InventorsRomain Chevrot, Fatoumata Tambadou, Thibault Caradec, Sandrine Didelot, Cyrille Barthelemy
Estimating the term…
PmxA synthetase involved in polymyxin E synthesis, comprising four adenylation sites, characterized in that the second adenylation site has at least 90% identity with the peptide sequence SEQ ID NO 1: VTEAEKADLLGRFNDTTTEFPRGKTLIQLFEEQVERIPDAAAITLNEQE LTYRELNERVNRLARTLRSHGISKGRLVAILAERSIEMVVGMLAAHKAG AAYVPIDPEYPEERIRFLIEDSGGQVMLTQSRLRERLAGSDPVILLDDE SFYHEDGTNLNTGIEATDLACVIYTSGTTGKPKGNPVSHRNIVRVVQNT NYIDITERDHVLQLSSYSFDGATFDIFGALTNGARLVLVPYETLLEIGR LADLIQRERISVMFITTAFFNILVDVNVDCLRDVRAILFGGERVSVGHV RKALAHIGPGRLNHVYGPTESTVYTTYLPVDFVDELAVTVPIGRPISNT TVYIVDSRNKLLPIGVAGELCVGGEGLVRGYNNRPELTAEKFVDNPFVP GERMYRTGDLAKWLPDGTIEYVGRTDDQVKIRGFRIELGEIEAQLQKVE GIRKTTVFARENASGEKQLCAYYEADCELPAAELKSVLSKELPAYMIPA YLIQLERLPLTTNGKVDRRSLPAPEESLQPGGG, the underlined amino acids DGFFLGVVYK being conserved and forming a binding pocket specific for a leucine, isoleucine or valine residue.
Method for producing polymyxin E, comprising culturing a microorganism transformed by introducing a gene encoding a PmxA synthetase in an appropriate mineral medium, and purifying, from the culture medium, the polymyxin E produced,
Method for producing polymyxin E, comprising culturing a microorganism transformed by introducing a gene encoding a PmxA synthetase in an appropriate mineral medium, and purifying, from the culture medium, the polymyxin E produced,
Method for producing polymyxin E variants, comprising culturing a microorganism transformed by introducing a gene encoding a PmxA synthetase in an appropriate mineral medium, and purifying, from the culture medium, the polymyxin E variant(s),
Dependencies are inferred from the claim text.