Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
AssigneeNovozymes, Inc.
InventorsMatthew Sweeney, Mark Wogulis
Life of this patent
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Abstract
The present invention relates to variants of GH61 polypeptides having cellulolytic enhancing activity, wherein the variants comprise a substitution at one or more positions corresponding to positions 90, 131, 134, and 141 of the full-length polypeptide of SEQ ID NO: 2, and have cellulolytic enhancing activity. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims27
A variant of a parent GH61 polypeptide having cellulolytic enhancing activity, comprising a substitution at one or more positions corresponding to positions 90, 131, 134, and 141 of the full-length polypeptide of SEQ ID NO:
- 2, wherein the substitution at a position corresponding to position 90 is with Val, the substitution at a position corresponding to position 131 is with Ser, the substitution at a position corresponding to position 134 is with Leu, and the substitution at a position corresponding to position 141 is with Trp, wherein the variant has cellulolytic enhancing activity and increased thermal activity and/or increased thermostability, and wherein the variant has
- at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2.
A method for obtaining a variant of a parent GH61 polypeptide having cellulolytic enhancing activity, comprising introducing into the parent GH61 polypeptide a substitution at one or more positions corresponding to positions 90, 131, 134, and 141 of the full-length polypeptide of SEQ ID NO:
- 2, wherein the substitution at a position corresponding to position 90 is with Val, the substitution at a position corresponding to position 131 is with Ser, the substitution at a position corresponding to position 134 is with Leu, and the substitution at a position corresponding to position 141 is with Trp, wherein the variant has cellulolytic enhancing activity and increased thermal activity and/or increased thermostability, and wherein the variant has
- at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.