Ketoreductase polypeptides

An engineered polypeptide having ketoreductase activity, wherein said engineered polypeptide comprises a region with an amino acid sequence having at least 90% sequence identity to residues 90 to 211 of SEQ ID NO:

• 130, comprising a substitution at position X190, and further comprising one or more of the following features selected from:
• residue corresponding to X94 is asparagine, glycine, serine, or a polar residue;
• residue corresponding to X95 is an aliphatic residue;
• residue corresponding to X96 is glutamine, asparagine, or threonine;
• residue corresponding to X101 is an acidic, non-polar, or a polar residue;
• residue corresponding to X105 is an acidic or non-polar residue;
• residue corresponding to X108 is a hydrophilic, polar or constrained residue;
• residue corresponding to X111 is a non-polar or aliphatic residue;
• residue corresponding to X112 is an acidic or polar residue;
• residue corresponding to X113 is a non-polar or aliphatic residue;
• residue corresponding to X117 is a non-polar or a polar residue;
• residue corresponding to X127 is a basic residue;
• residue corresponding to X147 is a non-polar, aliphatic, aromatic, or hydrophobic residue;
• residue corresponding to X152 is a non-polar, basic, or hydrophilic residue;
• residue corresponding to X157 is a polar residue;
• residue corresponding to X163 is a non-polar or aliphatic residue;
• residue corresponding to X176 is a non-polar or aliphatic residue;
• residue corresponding to X194 is a constrained, basic, or polar residue;
• residue corresponding to X197 is a hydrophilic, acidic, basic, aliphatic or a non-polar residue;
• residue corresponding to X198 is an acidic, basic, hydrophilic, or non-polar residue;
• residue corresponding to X199 is an acidic, aliphatic, or non-polar residue;
• residue corresponding to X200 is an acidic or constrained residue;
• residue corresponding to X202 is a non-polar residue;
• residue corresponding to X206 is a non-polar, aromatic, or hydrophobic residue;
• residue corresponding to X211 is a basic residue, and
• wherein the amino acid sequence can optionally have one or more differences at other amino acid residues in the domain as compared to the reference sequence.