Method and assembly for extraction of regenerative cellular components from adipose tissue

A method for extracting regenerative cellular components from adipose tissue comprising the steps of:

• a) filtering a harvested adipose tissue for recovering its liquid fraction by means of a filtering element having a first cut-off value between 40 microns and 70 microns;
• b) separating regenerative cellular components present in the liquid fraction by means of cell size segregation, which blocks cells bigger than a second cut-off value ranging from 8 to 20 microns;
• c) recovering the liquid regenerative cells-rich fraction comprising the regenerative cellular components not having being blocked during the separating step b);
• and
• d) separating regenerative cellular components present in a dense fraction filtered from the liquid fraction in step a).

A method for extracting regenerative cellular components from adipose tissue comprising the steps of:

• a) filtering a harvested adipose tissue for recovering its liquid fraction by means of a filtering element having a first cut-off value between 40 microns and 70 microns;
• b) separating regenerative cellular components present in the liquid fraction by means of cell size segregation, which blocks cells bigger than a second cut-off value ranging from 8 to 20 microns;
• and
• c) recovering the liquid regenerative cells-rich fraction comprising the regenerative cellular components not having being blocked during the separating step b);
• wherein steps a)-c) are carried out at room temperature.

An apparatus for extraction of regenerative cellular components from an adipose tissue comprising:

• a filtering element in a filtering pouch having a first inlet connector and a first outlet connector, the first inlet and first outlet connectors being air-contamination free, the filtering element having a first cut-off value between 40 microns and 70 microns for separating the liquid fraction from the dense fraction of a harvested adipose tissue;
• a separation device having a second cut-off value ranging from 8 to 20 microns;
• and
• a recovering chamber for collecting the regenerative cells-rich fraction comprising the regenerative cellular components not having being blocked by the separation device.