Method for producing pluripotent stem cell having improved chimera forming ability from primed pluripotent stem cell, and composition and combination therefor
AssigneeThe University of Tokyo
InventorsHiromitsu Nakauchi, Hideki Masaki, Hideyuki Murayama
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Abstract
The present invention provides a method for putting a primed pluripotent stem cell in a less differentiated state, a method for producing a pluripotent stem cell having an improved chimera forming ability from a primed pluripotent stem cell, and a method for producing a chimeric animal by using the pluripotent stem cell obtained by any of these methods. The present invention also provides a composition containing a Wnt/β catenin signal inhibitor.
Claims4
A method of increasing chimera forming ability of a primed pluripotent stem cell, comprising treating a primed pluripotent stem cell with a Wnt/β-catenin signal transduction selective inhibitor and a signal transducer and activator of transcription 3 (STAT3) activator to place the primed pluripotent stem cell in a state where a Wnt/β-catenin signal is selectively inhibited, among Wnt signaling pathways, and a STAT3 signal is activated, wherein following the treatment the primed pluripotent stem cell has increased chimera forming ability compared to an untreated control primed pluripotent stem cell.
Dependencies are inferred from the claim text.