Biochemical cartridge, and biochemical cartridge and cartridge holder set

A cartridge for sending a liquid inside a sealed space, the cartridge comprising:

• a cartridge main body having a top surface and a bottom surface substantially in one plane;
• a plurality of chambers internally provided in the cartridge main body that accept the liquid, including a first chamber having a first opening, a second chamber having a second opening and a third opening, and a third chamber having a fourth opening;
• and
• an elastic body having substantially planar top and bottom surfaces wherein one of the surfaces of the elastic body is attached to only first portions of the bottom surface of the cartridge main body among the first portions and second portions of the bottom surface of the cartridge main body,
• wherein the elastic body undergoes deformation with changes in externally applied pressure,
• wherein the elastic body is configured to close the bottom surface side of the plurality of chambers,
• wherein a part of the second portions of the bottom surface of the cartridge main body is between the first opening of the first chamber and the second opening of the second chamber and another part of the second portions of the bottom surface of the cartridge main body is between the third opening of the second chamber and the fourth opening of the third chamber,
• wherein the elastic body only deforms away from the substantially planar bottom surface of the cartridge main body at the second portions of the bottom surface of the cartridge main body wherein a flow channel is formed after the elastic body is deformed,
• wherein in a state without externally applied pressure causing deformation of the elastic body, substantially an entirety of the one surface of the elastic body contacts the bottom surface of the cartridge main body in the one plane,
• wherein at least the first chamber and the second chamber initially hold a reagent, and
• wherein a film is attached to the top surface of the cartridge main body covering each of the first chamber, the second chamber, and the third chamber.