De novo structural protein design for manufacturing high strength materials
AssigneeThe Penn State Research Foundation
InventorsMelik Demirel, Benjamin Allen, Huihun Jung
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Abstract
Provided is a supramolecular polypeptide comprising alternating repeats of crystallite-forming subsequences and amorphous subsequences. The crystallite-forming subsequences form crystallites comprising stacks of one or more β-sheets and the amorphous subsequences form a network of hydrogen bonds. The supramolecular polypeptides are capable of exhibiting self-healing behavior.
Claims1
A method of making a synthetic or recombinant polypeptide capable of forming at least a first crystal-forming domain block and at least a first amorphous domain block, the method comprising selecting an amino acid sequence that is capable of forming the first crystal domain block, wherein the crystal domain block is from about 2 nm to about 5 nm long and comprises from 10 to 30 amino acids, and selecting an amino acid sequence that is capable of forming a first amorphous domain block, wherein the first amorphous domain block is about 3 nm long and comprises about 15 amino acids, and forming the synthetic or recombinant polypeptide by incorporating [i] the amino acid sequence comprising AA[XXXXXX]HH (SEQ ID NO:
- 313), wherein the XXXXXX comprises histidine, threonine, valine, alanine, serine, or a combination thereof that is capable of forming the first crystal-forming domain block and [ii] the amino acid sequence comprising a glycine rich sequence according to Table 6 that is capable of forming the first amorphous domain block into the synthetic or recombinant polypeptide.
Dependencies are inferred from the claim text.