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US 10,246,485 B2Granted
Method to concentrate von willebrand factor or complexes thereof
AssigneeCSL Limited
InventorsHolger Lind, Sonja Beckmann-Scheld, Katharina Propp
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Abstract
The present invention relates to a method for concentrating von Willebrand Factor (VWF) from an aqueous solution comprising precipitating the VWF by providing calcium ions and phosphate ions and the subsequent resolubilization of the VWF by means of an aqueous solution comprising a calcium complexing compound.
Claims10
A method for concentrating von Willebrand Factor (VWF) from an aqueous solution comprising the steps of
- (a) precipitating the VWF by adding calcium ions and phosphate ions to the aqueous solution,
- (b) separating the precipitate formed in step (a), and
- (c) resolubilizing the precipitate isolated in step (b) by contacting the precipitate with an aqueous solution comprising a calcium complexing agent, wherein
- the VWF comprises a full-length VWF polypeptide.
1 more element
Dependencies are inferred from the claim text.