Method of preparation of nanopore and uses thereof
AssigneesThe Trustees of Columbia University in the City of New York, The United States of America as represented by the Secretary of Commerce
InventorsJingyue Ju, Shiv Kumar, Chuanjuan Tao, Minchen Chien, James J. Russo, John J. Kasianowicz, Joseph W. F. Robertson
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Abstract
This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.
Claims17
A tagged nucleotide, wherein the nucleotide comprises a tag capable of being cleaved in a nucleotide polymerization event and detected with the aid of a nanopore, wherein the tagged nucleotide has the structure:
- wherein the tag comprises an oligonucleotide which comprises nucleotides having natural bases and (i) at least one abasic site or (ii) at least one nucleotide having a modified base;
- wherein R1 is OH, wherein R2 is H or OH, wherein X is a linker and may comprise O, NH, S, or CH2, wherein Z is O, S, or BH3, wherein the base is adenine, guanine, cytosine, thymine, uracil, or a derivative of one of these bases, wherein n is 1, 2, 3, or 4.
Dependencies are inferred from the claim text.