Hydrazinyl and aminooxy compounds and their methods of use
AssigneeLife Technologies Corporation
InventorsWenjun Zhou, Kyle Gee, Yolanda Tennico, Peter Slade, Hee Chol Kang, Shaheer Khan, Brian Evans, James Stray
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Abstract
The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.
Claims32
A compound selected from the group consisting of:
- or a salt thereof.
A method for determining the presence of an analyte of interest in a sample, wherein the method comprises:
- a) contacting the sample with a compound selected from the group consisting of:
- or a salt thereof;
- b) incubating the sample and the compound or composition for a sufficient amount of time to form a complex between the compound and analyte;
- c) illuminating the complex with an appropriate wavelength to form an illuminated complex;
- and
- d) detecting emissions from the illuminated complex, thereby determining the presence of the analyte.
A method of detecting a glycan in a sample, the method comprising:
- a) contacting the sample with a [a] compound selected from the group consisting of:
- or a salt thereof;
- b) incubating the sample and the compound or composition for a sufficient amount of time to form a complex between the compound and glycan;
- c) illuminating the complex with an appropriate wavelength to form an illuminated complex;
- and
- d) detecting emissions from the illuminated complex, thereby determining the presence of the glycan.
Dependencies are inferred from the claim text.