US 10,245,590 B2 · High-speed real-time PCR device based on lab-on-a-chip for detecting food-borne bacteria to agrifood, and methods for detecting food-borne bacteria to agrifood using the same

Abstract

The present invention relates to an ultra-high speed real-time PCR device on the basis of a lab-on-a-chip for detecting bacteria that causes food poisoning pertaining to agricultural food and a food poisoning detection method using the same. The present invention can provide a micro PCR chip which can simultaneously accommodate a plurality of small-volume samples and concurrently secure maximum thermal contact efficiency with a heating block so as to secure rapid results, and accurately measure an optical signal emitted from a nucleic acid amplification product even without any separate filtering or processing. Further, on the basis of the PCR chip, the present invention can provide a real-time PCR device which can rapidly obtain a nucleic acid amplification result of which the reliability is secured even without a complicated light-signal measuring module.

Claims19

Dependent claims

A micro Polymerase Chain Reaction (PCR) chip, comprising:

a reaction chamber having an opened upper surface intended to receive a fluid;
and a cover intended to seal the reaction chamber, the cover comprising a light-transmitting portion of a light transmitting material which is faced with the opened upper surface of the PCR reaction chamber to close the open upper surface, the light-transmitting portion having a protruding part that extends toward the interior of the reaction chamber from regions of closed surfaces faced on the opened upper surface of the chamber, and the end of the protruding part being adjacent to or in contact with the surface of said fluid, wherein the protruding part of the light-transmitting portion is located in an optical path between a light-emitting module and a light-detecting module for detecting optical signals in said fluid.

Dependencies are inferred from the claim text.

Facts

Application

15/109,078Filed 30 Dec 2014

Priority

KR 10-2013-016868931 Dec 2013

Published

US 2017/0157614 A18 Jun 2017

Granted

2 Apr 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification15 codes

B01L 7/5255
With physical movement of samples between temperature zones: by moving sample containers
B01L 3/50851
For multiple samples, e.g. microtitration plates: specially adapted for heating or cooling samples
B01L 3/50853
For multiple samples, e.g. microtitration plates: with covers or lids
C12Q 1/686
Nucleic acid amplification reactions: polymerase chain reaction [PCR]

High-speed real-time PCR device based on lab-on-a-chip for detecting food-borne bacteria to agrifood, and methods for detecting food-borne bacteria to agrifood using the same

Life of this patent

High-speed real-time PCR device based on lab-on-a-chip for detecting food-borne bacteria to agrifood, and methods for detecting food-borne bacteria to agrifood using the same

Life of this patent

Abstract

Claims19