Glycosaminoglycans

A method of treatment of injury or disease, the method comprising administering, to an individual in need thereof, a therapeutically effective amount of an isolated or substantially purified heparan sulphate composition comprising a heparan sulphate component, wherein the heparan sulphate component is at least 97% HS/BMP2 which is capable of specific binding to SEQ ID NO:1 or 6, and to BMP2, and which is obtained by a method comprising:

• (i) providing a solid support having polypeptide molecules adhered to the support, wherein the polypeptide consists of the amino acid sequence QAKHKQRKRLKSSCKRHP [SEQ ID NO:1] or QAKHKQRKRLKSSCKRH [SEQ ID NO:6];
• (ii) contacting the polypeptide molecules with a heparan sulphate fraction obtained from mammalian tissue or extracellular matrix such that polypeptide-heparan sulphate complexes are allowed to form;
• (iii) partitioning polypeptide-heparan sulphate complexes from the remainder of the mixture;
• (iv) dissociating the heparan sulphate from the polypeptide-heparan sulphate complexes by disrupting the polypeptide-heparan sulphate complexes;
• and
• (v) collecting the dissociated heparan sulphate;
• wherein the method comprises the repair and/or regeneration of bone.