Constitutively active PYR/PYL receptor proteins for improving plant stress tolerance
AssigneeThe Regents of the University of California
InventorSean R. Cutler
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Abstract
The present invention provides methods of regulating plant stress tolerance.
Claims22
An expression cassette comprising a heterologous promoter operably linked to a polynucleotide encoding a mutated PYR/PYL receptor polypeptide, wherein the mutated PYR/PYL receptor polypeptide is at least 90% identical to any of SEQ ID NO:85, SEQ ID NO:87, SEQ ID NO:88, SEQ ID NO:89, or SEQ ID NO:90, and further comprises an amino acid substitution, relative to a naturally-occurring PYR/PYL receptor polypeptide, at position H60 as numbered with reference to SEQ ID NO:89, wherein the naturally-occurring PYR/PYL receptor polypeptide does not inhibit a type 2 protein phosphatase (PP2C) in the absence of abscisic acid (ABA) and wherein the mutated PYR/PYL receptor polypeptide inhibits the PP2C in the absence of ABA or an ABA agonist.
A plant comprising a heterologous expression cassette, the expression cassette comprising a heterologous promoter operably linked to a polynucleotide encoding a mutated PYR/PYL receptor polypeptide, wherein the mutated PYR/PYL, receptor polypeptide is at least 90% identical to any of SEQ ID NO:85, SEQ ID NO:87, SEQ ID NO:88, SEQ ID NO:89, or SEQ ID NO:90 and further comprises an amino acid substitution, relative to a naturally-occurring PYR/PYL receptor polypeptide, at position H60 as numbered with reference to SEQ ID NO:89, wherein the naturally-occurring PYR/PYL receptor polypeptide does not inhibit a type 2 protein phosphatase (PP2C) in the absence of abscisic acid (ABA) and wherein the mutated PYR/PYL receptor polypeptide inhibits the PP2C in the absence of ABA or an ABA agonist.
Dependencies are inferred from the claim text.