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US 10,221,401 B2Granted
Oxygen tolerant hydrogenase by mutating electron supply pathway
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsJames Robert Swartz, Jamin Koo
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Abstract
Compositions and methods are provided for an O2 tolerant Fe—Fe hydrogenase. The hydrogenases of the invention comprise specific amino acid substitutions relative to the native, or wild-type enzymes.
Claims6
A modified Fe—Fe hydrogenase comprising at least one amino acid substitution relative to the wild-type sequence, said modified Fe—Fe hydrogenase has
- [i] at least 80% sequence identity to SEQ ID NO:
- 1, and at least one amino acid substitution at L192, G194, T356 or S357;
- [ii] at least 96% sequence identity to SEQ ID NO:
- 2, and at least one amino acid substitution at L191, G193, N355 and/or D356;
- [iii] at least 80% sequence identity to SEQ ID NO:
- 3, and at least one amino acid substitution at L192, G194, T356 or A357;
- [iv] at least 80% sequence identity to SEQ ID NO:
- 4, and at least one amino acid substitution at L192, G194, T356 or A357;
- or
- [v] at least 80% sequence identity to SEQ ID NO:
- 5, and at least one amino acid substitution at L191, G193, N355 or D356;
- wherein the modified enzyme retains at least 10% of the initial specific activity following exposure to 0.01 atm. O2 for 5 minutes.
9 more elements
Dependencies are inferred from the claim text.