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US 10,145,833 B2Granted
Detection method
AssigneeConidia Bioscience Limited
InventorJoan Kelley
Life of this patent
Estimating the term…
Abstract
A method of analysis of a hydrocarbon fuel for the presence of a micro-organism comprises contacting a fuel sample with an aqueous diluent and with an antibody reactive with the micro-organism, or reactive with a metabolite or breakdown product produced by the micro-organism, to detect the presence or absence of the micro-organism.
Claims12
A method of analysis of a sample of a liquid hydrocarbon fuel for the presence of Hormoconis resinae, the method comprising the steps of:
- 1) mixing the liquid fuel sample with an aqueous diluent containing an emulsifier;
- 2) contacting the mixture or one phase thereof with an antiserum comprising antibodies raised against a homogenized preparation of Hormoconis resinae, wherein the Hormoconis resinae in the homogenized preparation was grown in contact with the liquid hydrocarbon fuel;
- and
- 3) detecting the presence or absence of bound antibodies in the sample,
- wherein the method provides a result within two hours from step 1, and wherein the detection of bound antibodies indicates the presence of Hormoconis resinae which is actively growing in liquid hydrocarbon fuel.
2 more elements
A kit for analyzing a sample of a liquid hydrocarbon fuel for the presence of Hormoconis resinae, the kit comprising:
- 1) a homogenized preparation of Hormoconis resinae, wherein the Hormoconis resinae in the homogenized preparation was grown in contact with liquid hydrocarbon fuel;
- 2) an antiserum comprising antibodies raised against a homogenized preparation of Hormoconis resinae, wherein the Hormoconis resinae in the homogenized preparation was grown in contact with the liquid hydrocarbon fuel;
- and
- 3) an aqueous diluent containing an emulsifier.
1 more element
Dependencies are inferred from the claim text.