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US 10,119,969 B2Granted
Compositions for the detection of microbial contaminants
AssigneeCharles River Laboratories, Inc.
InventorsNorman R. Wainwright, Foster T. Jordan
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AssigneeCharles River Laboratories, Inc.
InventorsNorman R. Wainwright, Foster T. Jordan
Estimating the term…
The invention provides methods and compositions for the detection and/or quantification of a microbial contaminant, for example, a bacterial endotoxin or a glucan, in a sample. In particular, the invention provides a test cartridge useful in the practice of hemocyte lysate-based assays for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides methods of making and using such cartridges. In addition, the invention provides a rapid, sensitive, multi-step kinetic hemocyte lysate-based assay for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides a glucan-specific lysate that can be used in a variety of assay formats, including, for example, a test cartridge, optionally configured to perform a kinetic assay.
A manufacture comprising a solid support having dried thereon a composition comprising a mixture of a hemocyte lysate and (i) an anti-frothing agent, (ii) a resolubilizing agent, (iii) an anti-flaking agent and an anti-frothing agent, (iv) an anti-flaking agent and a resolubilizing agent, (v) an anti-frothing agent and a resolubilizing agent, or (vi) an anti-flaking agent, an anti-frothing agent and a resolubilizing agent, wherein said lysate is capable of being solubilized or reconstituted by a sample.
A manufacture comprising a solid support comprising glass or plastic having dried thereon a composition comprising mixture of a hemocyte a lysate and (i) an anti-flaking agent, (ii) an anti-frothing agent, (iii) a resolubilizing agent, (iv) an anti-flaking agent and an anti-frothing agent, (v) an anti-flaking agent and a resolubilizing agent, (vi) an anti-frothing agent and a resolubilizing agent, or (vii) an anti-flaking agent, an anti-frothing agent and a resolubilizing agent, wherein said lysate is capable of being solubilized or reconstituted by a sample.
Dependencies are inferred from the claim text.